Study of Genetic Diversity among Philodendron Varieties by Randomly Amplified Polymorphic DNA Markers

نویسندگان

  • Achar Devaraja AM Biotechnology laboratory, Indo American Hybrid Seeds (I) Pvt Ltd., Bangalore 560 098, India
  • Jakeer P Biotechnology laboratory, Indo American Hybrid Seeds (I) Pvt Ltd., Bangalore 560 098, India
  • Jakhar Mamta Biotechnology laboratory, Indo American Hybrid Seeds (I) Pvt Ltd., Bangalore 560 098, India
  • Shetty KPV Biotechnology laboratory, Indo American Hybrid Seeds (I) Pvt Ltd., Bangalore 560 098, India
چکیده مقاله:

In the present study, Randomly Amplified Polymorphic DNA (RAPD) markers were used to analyze the genetic diversity in 20 varieties of Philodendron. The polymerase chain reaction was performed with 60 RAPD primers, out of which 21 primers showed clear amplification as well as more polymorphism. In total, 354 scorable RAPD loci with 348 polymorphic bands (98%) were observed. Percentages of polymorphic bands ranged from 80% to 100%. Amplified DNA fragments were used for statistical analysis. The data was obtained by scoring the bands and analyzed by using STATISTICA software. A dendrogram was obtained by cluster analysis based on the presence or absence of band which indicates similarity and diversities. The cluster analysis grouped the Philodendron varieties based on the growth habits, morphological characters and also geographical origin. This indicates that there is an association between the RAPD patterns and the geographic origin of the varieties used. The genetic diversity among the varieties was moderate, which might be due to genetically heterozygous and somaclonal variation.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

study of genetic diversity among philodendron varieties by randomly amplified polymorphic dna markers

in the present study, randomly amplified polymorphic dna (rapd) markers were used to analyze the genetic diversity in 20 varieties of philodendron. the polymerase chain reaction was performed with 60 rapd primers, out of which 21 primers showed clear amplification as well as more polymorphism. in total, 354 scorable rapd loci with 348 polymorphic bands (98%) were observed. percentages of polymo...

متن کامل

Genetic diversity among Elaeagnus angustifolia L. populations based on some morphological traits and Random Amplified Polymorphic DNA Markers

Elaeagnus angustifolia L. is a Eurasian tree that has become naturalized and has various ecological, medicinal and economical uses. In this study, a combination of morphological traits and RAPD markers were used to study the presence or absence of an association between genetic variation and morphological features among five populations of E. angustifolia collected from the East Azarbaijan of I...

متن کامل

Evaluation of Genetic Variability in a Breeder Flock of Native Chicken Based on Randomly Amplified Polymorphic DNA Markers

A study was undertaken to evaluate the genetic variation in the 10th generation of a breeder flock of native breed selected for high egg and meat production in native fowls breeding station, Mazandaran, Iran. Venous blood samples were collected from 100 birds of both sexes. The RAPD-PCR technique was applied to generate a DNA fingerprint of individuals. Initially, a total of 20 ten-nucleotide a...

متن کامل

Comparison of Hemp Varieties Using Random Amplified Polymorphic DNA Markers

to allow cultivation of hemp varieties with a 9–THC content below 0.3%. By 1998 hemp was reintroduced The objective was to study the genetic structure and degree of in practically all of Europe (Bócsa and Karus, 1998), variability of hemp (Cannabis sativa L.) varieties. Six varieties of hemp were analyzed by random amplified polymorphic DNA though in some countries significant resources are sti...

متن کامل

Study of Genetic Diversity in Wheat (triticum Aestivum L.) Using Random Amplified Polymorphic Dna (rapd) Markers

Twelve wheat genotypes developed through hybridization programme were screened for genetic diversity through RAPD marker. A total of 102 loci were amplified with 14 primers out of which 91 (89.2%) were polymorphic and only 11(10.8%) were monomorphic. Fragments size ranged from 142bp-5.3kb and fragments produced by various primers ranged from 1-11 with an average of 7.1 fragments per primer. The...

متن کامل

Use of randomly amplified polymorphic DNA markers for the genetic analysis of relatedness and diversity in chickens and turkeys.

A study involving the use of random amplified polymorphic DNA (RAPD) was conducted to evaluate genetic polymorphism and relatedness within and among four chicken breeds: Araucona, Rhode Island Red, White Leghorn, and White Plymouth Rock, and two turkey populations, a long-term randombred and a commercial strain. A total of 60 random primers were used in the RAPD analyses. Forty-two of the 60 pr...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ذخیره در منابع من قبلا به منابع من ذحیره شده

{@ msg_add @}


عنوان ژورنال

دوره 4  شماره 1

صفحات  19- 26

تاریخ انتشار 2014-03-01

با دنبال کردن یک ژورنال هنگامی که شماره جدید این ژورنال منتشر می شود به شما از طریق ایمیل اطلاع داده می شود.

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023